Publication Date:
1984-08-10
Description:
Mung bean nuclease was found to cut the genomic DNA of the malaria parasite Plasmodium at positions before and after genes but not within gene-coding regions. This cleavage, which had nearly the preciseness of a restriction nuclease, required controlled conditions in the presence of formamide. Southern blot analysis showed that the coding areas for Plasmodium actin, circumsporozoite protein, histidine-rich protein, ribosomal RNA's, and tubulin are each cleaved from genomic DNA to yield a single major band on an agarose gel. DNA sequence data on several clones of mung bean nuclease cleavage products containing the gene for the circumsporozoite protein of Plasmodium falciparum confirmed that cleavage sites are before and after genes. Recognition and cleavage of DNA did not seem to be related to any primary sequence but may be related to structural features of the DNA duplex that demarcate genes. Mung bean nuclease-cleaved DNA could be inserted directly into a lambda expression vector, yielding a representative but small gene bank of intact gene fragments.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McCutchan, T F -- Hansen, J L -- Dame, J B -- Mullins, J A -- New York, N.Y. -- Science. 1984 Aug 10;225(4662):625-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6330899" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Monoclonal/metabolism
;
Antigens, Surface/genetics
;
DNA/isolation & purification/*metabolism
;
Electrophoresis, Agar Gel
;
Endonucleases/*metabolism
;
*Genes
;
Macaca mulatta
;
Plasmodium falciparum/*genetics
;
*Protozoan Proteins
;
Single-Strand Specific DNA and RNA Endonucleases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
