Publication Date:
1981-09-04
Description:
The arrangement of the human insulin gene in DNA from 87 individuals was analyzed by the Southern blot hybridization technique with a cloned genomic human insulin probe. Insertions of 1.5 to 3.4 kilobase pairs in the 5'-flanking region of the gene were found in DNA from 38 individuals. These insertions occurred within 1.3 kilobase pairs of the transcription initiation site. In contrast, no insertions were observed in the region 3' to the coding sequence. The prevalence of these insertions in type 2 diabetes was significantly greater than in the other groups (P less than .001). The limitation of this striking length polymorphism to a potential promoter region suggests that these insertions may play a role in insulin gene expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rotwein, P -- Chyn, R -- Chirgwin, J -- Cordell, B -- Goodman, H M -- Permut, M A -- AM-00033/AM/NIADDK NIH HHS/ -- AM-07120/AM/NIADDK NIH HHS/ -- AM-16724/AM/NIADDK NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1981 Sep 4;213(4512):1117-20.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6267694" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
DNA Restriction Enzymes
;
Diabetes Mellitus/*genetics
;
Gene Expression Regulation
;
Genes
;
Genetic Linkage
;
Humans
;
Insulin/*genetics
;
Leukocytes
;
Operon
;
Polymorphism, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
