Publication Date:
1989-03-10
Description:
Tension and intracellular free calcium concentration [( Ca2+]i) were measured simultaneously in single smooth muscle cells isolated from the anterior byssus retractor muscle (ABRM) of Mytilus edulis that were loaded with the fluorescent Ca2+ indicator fura-2. Electrical stimulation evoked a transient elevation of [Ca2+]i associated with a "catch" contraction. During the catch state, however, [Ca2+]i was effectively at its resting level and was unaffected by 5-hydroxytryptamine, which induced a rapid relaxation from catch. The results indicate that a maintained high [Ca2+]i is not required for the maintenance of catch tension in intact ABRM and that there was no significant change in [Ca2+]i upon abolition of catch.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ishii, N -- Simpson, A W -- Ashley, C C -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 1989 Mar 10;243(4896):1367-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Zoological Institute, Faculty of Science, University of Tokyo, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2922614" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Benzofurans
;
Bivalvia
;
Calcium/*physiology
;
Fluorescent Dyes
;
Fura-2
;
In Vitro Techniques
;
*Muscle Contraction
;
Muscle, Smooth/*physiology
;
Spectrometry, Fluorescence/instrumentation/methods
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
