Publication Date:
1989-04-14
Description:
A monoclonal antibody was used to show directly positive thymic selection of the T cell repertoire in mouse strains expressing the 17a beta-chain variable domain (V beta 17a) of the T cell receptor. In the absence of the potent tolerizing class II major histocompatibility complex (MHC) molecule, I-E, peripheral expression of V beta 17a+ T cell receptors varied with the MHC haplotype of the mouse strain. In the most extreme case, H-2q mice expressed high peripheral levels of CD4+ V beta 17a+ T cells (14 to 19 percent), whereas H-2b mice expressed low levels (3 to 4 percent). Analysis of (b x q)F1 mice and chimeric mice showed that these differences were determined by positive thymic selection and implicated the thymic epithelium as the controlling cell type.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Blackman, M A -- Marrack, P -- Kappler, J -- New York, N.Y. -- Science. 1989 Apr 14;244(4901):214-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Denver, CO.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2784868" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Monoclonal/immunology
;
Antigens, Differentiation, T-Lymphocyte/immunology
;
Chimera
;
H-2 Antigens/genetics/immunology
;
Haplotypes
;
Immunoglobulin Variable Region/genetics/immunology
;
*Major Histocompatibility Complex
;
Mice
;
T-Lymphocytes/*immunology
;
Thymus Gland/*immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
