Publication Date:
1989-06-23
Description:
An airway epithelial cell line (CF/T43) was developed by infecting cultured airway epithelial cells from patients with cystic fibrosis (CF) with the pZIPneoSV(X)1/SV40T retrovirus and selecting for G418 resistance and ion transport properties. The distinctive chloride secretory phenotypes of the CF cell line CF/T43 and a normal cell line (NL/T4) were not perturbed by SV40T-induced cell transformation. Epithelial cell lines generated from CF cells with the SV40T gene can be used to test candidate CF genes and to evaluate the molecular mechanisms responsible for the CF phenotype.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jetten, A M -- Yankaskas, J R -- Stutts, M J -- Willumsen, N J -- Boucher, R C -- HL41983/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1989 Jun 23;244(4911):1472-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2472008" target="_blank"〉PubMed〈/a〉
Keywords:
Amiloride/pharmacology
;
Antigens, Polyomavirus Transforming/*genetics
;
Calcimycin/pharmacology
;
Cell Line
;
Cell Membrane/physiology
;
Chloride Channels
;
Chlorides/*physiology
;
Colforsin/pharmacology
;
Cystic Fibrosis/pathology/*physiopathology
;
Electric Conductivity
;
Epithelium/drug effects/pathology/physiology
;
Ethers/pharmacology
;
Freeze Fracturing
;
Humans
;
Intercellular Junctions
;
Ion Channels/physiology
;
Ionomycin
;
Membrane Proteins/*physiology
;
Microscopy, Electron
;
Nasal Polyps
;
Simian virus 40/*immunology
;
*Transformation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
