Publication Date:
1992-01-17
Description:
An immunodominant determinant for cytotoxic T lymphocytes (CTLs) exists in the hypervariable portion of human immunodeficiency virus-1 (HIV-1) gp160. Three mouse CTL lines (specific for isolates MN, RF, and IIIB) were examined for recognition of homologous determinants from distinct isolates. Only MN-elicited CTLs showed extensive interisolate cross-reactivity. Residue 325 played a critical role in specificity, with MN-elicited CTLs responding to peptides with an aromatic or cyclic residue and IIIB-induced cells recognizing peptides with an aliphatic residue at this position. CTL populations with broad specificities were generated by restimulation of IIIB-gp160 primed cells with MN-type peptides that have an aliphatic substitution at 325. This represents an approach to synthetic vaccines that can generate broadly cross-reactive CTLs capable of effector function against a wide range of HIV isolates.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Takahashi, H -- Nakagawa, Y -- Pendleton, C D -- Houghten, R A -- Yokomuro, K -- Germain, R N -- Berzofsky, J A -- New York, N.Y. -- Science. 1992 Jan 17;255(5042):333-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1372448" target="_blank"〉PubMed〈/a〉
Keywords:
AIDS Vaccines/immunology
;
Amino Acid Sequence
;
Animals
;
Cell Line
;
Cross Reactions
;
Epitopes/immunology
;
Gene Products, env/genetics/*immunology
;
HIV Antigens/immunology
;
HIV Envelope Protein gp160
;
HIV-1/*immunology
;
In Vitro Techniques
;
Mice
;
Mice, Inbred BALB C
;
Molecular Sequence Data
;
Protein Precursors/genetics/*immunology
;
Sequence Homology, Nucleic Acid
;
T-Lymphocytes, Cytotoxic/*immunology
;
Vaccination
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
