Publication Date:
2000-02-26
Description:
Steroid receptors bind to site-specific response elements in chromatin and modulate gene expression in a hormone-dependent fashion. With the use of a tandem array of mouse mammary tumor virus reporter elements and a form of glucocorticoid receptor labeled with green fluorescent protein, targeting of the receptor to response elements in live mouse cells was observed. Photobleaching experiments provide direct evidence that the hormone-occupied receptor undergoes rapid exchange between chromatin and the nucleoplasmic compartment. Thus, the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McNally, J G -- Muller, W G -- Walker, D -- Wolford, R -- Hager, G L -- New York, N.Y. -- Science. 2000 Feb 18;287(5456):1262-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, Bethesda, MD 20892-5055, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10678832" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Binding Sites
;
Cell Line, Transformed
;
Cell Nucleus/metabolism
;
Chromatin/*metabolism
;
Dexamethasone/metabolism/*pharmacology
;
Green Fluorescent Proteins
;
In Situ Hybridization, Fluorescence
;
Ligands
;
Luminescent Proteins
;
Mammary Tumor Virus, Mouse/genetics
;
Mice
;
Microscopy, Confocal
;
Microscopy, Fluorescence
;
Nucleosomes/metabolism
;
Receptors, Glucocorticoid/*metabolism
;
*Response Elements
;
*Terminal Repeat Sequences
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
