Publication Date:
2000-02-11
Description:
The roles of phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC) in chemoattractant-elicited responses were studied in mice lacking these key enzymes. PI3Kgamma was required for chemoattractant-induced production of phosphatidylinositol 3,4,5-trisphosphate [PtdIns (3,4,5)P3] and has an important role in chemoattractant-induced superoxide production and chemotaxis in mouse neutrophils and in production of T cell-independent antigen-specific antibodies composed of the immunoglobulin lambda light chain (TI-IglambdaL). The study of the mice lacking PLC-beta2 and -beta3 revealed that the PLC pathways have an important role in chemoattractant-mediated production of superoxide and regulation of protein kinases, but not chemotaxis. The PLC pathways also appear to inhibit the chemotactic activity induced by certain chemoattractants and to suppress TI-IglambdaL production.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Li, Z -- Jiang, H -- Xie, W -- Zhang, Z -- Smrcka, A V -- Wu, D -- New York, N.Y. -- Science. 2000 Feb 11;287(5455):1046-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, University of Rochester, Rochester, NY 14642, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10669417" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
B-Lymphocytes/immunology
;
Chemokine CCL4
;
Chemotactic Factors/*pharmacology
;
Chemotaxis, Leukocyte/*physiology
;
Immunoglobulin lambda-Chains/biosynthesis
;
Isoenzymes/*metabolism
;
Macrophage Inflammatory Proteins/pharmacology
;
Mice
;
N-Formylmethionine Leucyl-Phenylalanine/pharmacology
;
Neutrophil Infiltration
;
Neutrophils/metabolism/*physiology
;
Peritonitis/immunology
;
Phosphatidylinositol 3-Kinases/*metabolism
;
Phosphatidylinositol Phosphates/metabolism
;
Phospholipase C beta
;
Phosphorylation
;
*Signal Transduction
;
Skin Ulcer/pathology
;
Superoxides/metabolism
;
Type C Phospholipases/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
