Publication Date:
1999-12-22
Description:
The SGS1 gene of the yeast Saccharomyces cerevisiae encodes a DNA helicase with homology to the human Bloom's syndrome gene BLM and the Werner's syndrome gene WRN. The SRS2 gene of yeast also encodes a DNA helicase. Simultaneous deletion of SGS1 and SRS2 is lethal in yeast. Here, using a conditional mutation of SGS1, it is shown that DNA replication and RNA polymerase I transcription are drastically inhibited in the srs2Delta sgs1-ts strain at the restrictive temperature. Thus, SGS1 and SRS2 function in DNA replication and RNA polymerase I transcription. These functions may contribute to the various defects observed in Werner's and Bloom's syndromes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lee, S K -- Johnson, R E -- Yu, S L -- Prakash, L -- Prakash, S -- CA80882/CA/NCI NIH HHS/ -- GM19261/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1999 Dec 17;286(5448):2339-42.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10600744" target="_blank"〉PubMed〈/a〉
Keywords:
Bloom Syndrome/genetics
;
Codon
;
DNA Helicases/genetics/*physiology
;
*DNA Replication
;
DNA, Fungal/biosynthesis
;
Fungal Proteins/genetics/*physiology
;
Gene Deletion
;
Genes, Fungal
;
Humans
;
Mutation
;
RNA Polymerase I/metabolism
;
RNA Polymerase II/metabolism
;
RNA Polymerase III/metabolism
;
RNA, Fungal/biosynthesis
;
RNA, Messenger/biosynthesis/genetics
;
RNA, Ribosomal/biosynthesis
;
RNA, Transfer, Amino Acid-Specific/biosynthesis
;
RecQ Helicases
;
Saccharomyces cerevisiae/*genetics/metabolism
;
*Saccharomyces cerevisiae Proteins
;
*Transcription, Genetic
;
Werner Syndrome/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
