Publication Date:
1999-05-29
Description:
Inositol 1,4,5-trisphosphate (IP3) is a second messenger that elicits complex spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green fluorescent protein-tagged pleckstrin homology domain translocated from the plasma membrane to the cytoplasm in response to increased concentration of IP3. The detection of translocation enabled monitoring of IP3 concentration changes within single cells and revealed spatiotemporal dynamics in the concentration of IP3 synchronous with Ca2+ oscillations and intracellular and intercellular IP3 waves that accompanied Ca2+ waves. Such changes in IP3 concentration may be fundamental to Ca2+ signaling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hirose, K -- Kadowaki, S -- Tanabe, M -- Takeshima, H -- Iino, M -- New York, N.Y. -- Science. 1999 May 28;284(5419):1527-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, Faculty of Medicine, University of Tokyo and CREST, Japan Science and Technology Corporation, Tokyo 113-8654, Japan. hirose@calcium.cmp.m.u-tokyo.ac.jp〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10348740" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/pharmacology
;
Animals
;
Calcium/*metabolism
;
*Calcium Signaling
;
Cell Line
;
Cell Membrane/metabolism
;
Cell Nucleus/metabolism
;
Cytoplasm/metabolism
;
Dogs
;
Green Fluorescent Proteins
;
Inositol 1,4,5-Trisphosphate/*metabolism
;
Inositol Phosphates/metabolism
;
Isoenzymes/chemistry/metabolism
;
Ligands
;
Luminescent Proteins
;
Microscopy, Confocal
;
Phosphatidylinositol 4,5-Diphosphate/metabolism
;
Phospholipase C delta
;
Recombinant Fusion Proteins/metabolism
;
Time Factors
;
Type C Phospholipases/chemistry/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
