Publication Date:
1999-05-13
Description:
Transcription factors are often phosphorylated at multiple sites. Here it is shown that multiple phosphorylation sites on the budding yeast transcription factor Pho4 play distinct and separable roles in regulating the factor's activity. Phosphorylation of Pho4 at two sites promotes the factor's nuclear export and phosphorylation at a third site inhibits its nuclear import. Phosphorylation of a fourth site blocks the interaction of Pho4 with the transcription factor Pho2. Multiple phosphorylation sites provide overlapping and partially redundant layers of regulation that function to efficiently control the activity of Pho4.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Komeili, A -- O'Shea, E K -- New York, N.Y. -- Science. 1999 May 7;284(5416):977-80.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉University of California-San Francisco, Department of Biochemistry and Biophysics, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10320381" target="_blank"〉PubMed〈/a〉
Keywords:
Acid Phosphatase/metabolism
;
Amino Acid Substitution
;
Cell Nucleus/*metabolism
;
Cyclin-Dependent Kinases/metabolism
;
Cyclins/metabolism
;
*DNA-Binding Proteins
;
Fungal Proteins/genetics/*metabolism
;
*Homeodomain Proteins
;
Karyopherins
;
*Membrane Transport Proteins
;
Nuclear Localization Signals
;
Phosphorylation
;
Receptors, Cytoplasmic and Nuclear/metabolism
;
Recombinant Fusion Proteins/metabolism
;
*Repressor Proteins
;
Saccharomyces cerevisiae/genetics/*metabolism
;
*Saccharomyces cerevisiae Proteins
;
Trans-Activators/metabolism
;
Transcription Factors/genetics/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
