Publication Date:
1999-04-24
Description:
Control of cyclin levels is critical for proper cell cycle regulation. In yeast, the stability of the G1 cyclin Cln1 is controlled by phosphorylation-dependent ubiquitination. Here it is shown that this reaction can be reconstituted in vitro with an SCF E3 ubiquitin ligase complex. Phosphorylated Cln1 was ubiquitinated by SCF (Skp1-Cdc53-F-box protein) complexes containing the F-box protein Grr1, Rbx1, and the E2 Cdc34. Rbx1 promotes association of Cdc34 with Cdc53 and stimulates Cdc34 auto-ubiquitination in the context of Cdc53 or SCF complexes. Rbx1, which is also a component of the von Hippel-Lindau tumor suppressor complex, may define a previously unrecognized class of E3-associated proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Skowyra, D -- Koepp, D M -- Kamura, T -- Conrad, M N -- Conaway, R C -- Conaway, J W -- Elledge, S J -- Harper, J W -- AG11085/AG/NIA NIH HHS/ -- GM41628/GM/NIGMS NIH HHS/ -- GM54137/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1999 Apr 23;284(5414):662-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Verna and Marrs McLean Department of Biochemistry, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10213692" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Anaphase-Promoting Complex-Cyclosome
;
Animals
;
Carrier Proteins/chemistry/*metabolism
;
Cell Cycle Proteins/metabolism
;
Cell Line
;
*Cullin Proteins
;
Cyclins/*metabolism
;
F-Box Proteins
;
Fungal Proteins/*metabolism
;
Ligases/metabolism
;
Molecular Sequence Data
;
Peptide Synthases/*metabolism
;
Phosphorylation
;
Recombinant Fusion Proteins/metabolism
;
S-Phase Kinase-Associated Proteins
;
SKP Cullin F-Box Protein Ligases
;
Saccharomyces cerevisiae/metabolism
;
*Saccharomyces cerevisiae Proteins
;
Sequence Alignment
;
Ubiquitin-Conjugating Enzymes
;
*Ubiquitin-Protein Ligase Complexes
;
Ubiquitin-Protein Ligases
;
Ubiquitins/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
