Publication Date:
2014-07-19
Description:
Unfertilized oocytes have the intrinsic capacity to remodel sperm and the nuclei of somatic cells. The discoveries that cells can change their phenotype from differentiated to embryonic state using oocytes or specific transcription factors have been recognized as two major breakthroughs in the biomedical field. Here, we show that ASF1A, a histone-remodeling chaperone specifically enriched in the metaphase II human oocyte, is necessary for reprogramming of human adult dermal fibroblasts (hADFs) into undifferentiated induced pluripotent stem cell. We also show that overexpression of just ASF1A and OCT4 in hADFs exposed to the oocyte-specific paracrine growth factor GDF9 can reprogram hADFs into pluripotent cells. Our Report underscores the importance of studying the unfertilized MII oocyte as a means to understand the molecular pathways governing somatic cell reprogramming.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gonzalez-Munoz, Elena -- Arboleda-Estudillo, Yohanna -- Otu, Hasan H -- Cibelli, Jose B -- New York, N.Y. -- Science. 2014 Aug 15;345(6198):822-5. doi: 10.1126/science.1254745. Epub 2014 Jul 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉LARCEL, Laboratorio Andaluz de Reprogramacion Celular, BIONAND, Centro Andaluz de Nanomedicina y Biotecnologia Andalucia, 29590, Spain. ; Department of Genetics and Bioengineering, Istanbul Bilgi University 34060, Istanbul, Turkey. Department of Electrical Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA. ; LARCEL, Laboratorio Andaluz de Reprogramacion Celular, BIONAND, Centro Andaluz de Nanomedicina y Biotecnologia Andalucia, 29590, Spain. Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA. Department of Physiology, Michigan State University, East Lansing, MI 48824, USA. cibelli@msu.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25035411" target="_blank"〉PubMed〈/a〉
Keywords:
Acetylation
;
Cell Cycle Proteins/genetics/*metabolism
;
Cell Dedifferentiation
;
Cell Differentiation
;
*Cellular Reprogramming
;
Embryonic Stem Cells/cytology/physiology
;
Fibroblasts/cytology/physiology
;
Growth Differentiation Factor 9/metabolism
;
Histone Chaperones/genetics/*metabolism
;
Histones/metabolism
;
Humans
;
Induced Pluripotent Stem Cells/*physiology
;
Metaphase
;
Octamer Transcription Factor-3/metabolism
;
Oocytes/cytology/physiology
;
Signal Transduction
;
Transcriptional Activation
;
Transcriptome
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
