Publication Date:
2014-02-08
Description:
The distance between Ca(2+) channels and release sensors determines the speed and efficacy of synaptic transmission. Tight "nanodomain" channel-sensor coupling initiates transmitter release at synapses in the mature brain, whereas loose "microdomain" coupling appears restricted to early developmental stages. To probe the coupling configuration at a plastic synapse in the mature central nervous system, we performed paired recordings between mossy fiber terminals and CA3 pyramidal neurons in rat hippocampus. Millimolar concentrations of both the fast Ca(2+) chelator BAPTA [1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid] and the slow chelator EGTA efficiently suppressed transmitter release, indicating loose coupling between Ca(2+) channels and release sensors. Loose coupling enabled the control of initial release probability by fast endogenous Ca(2+) buffers and the generation of facilitation by buffer saturation. Thus, loose coupling provides the molecular framework for presynaptic plasticity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vyleta, Nicholas P -- Jonas, Peter -- New York, N.Y. -- Science. 2014 Feb 7;343(6171):665-70. doi: 10.1126/science.1244811.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉IST Austria (Institute of Science and Technology Austria), Am Campus 1, A-3400 Klosterneuburg, Austria.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24503854" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
CA3 Region, Hippocampal/metabolism/physiology
;
Calcium Channels/*metabolism
;
Chelating Agents/pharmacology
;
Egtazic Acid/analogs & derivatives/pharmacology
;
Hippocampus/drug effects/metabolism/*physiology
;
Mossy Fibers, Hippocampal/drug effects/metabolism/physiology
;
Neuronal Plasticity/drug effects/*physiology
;
Rats
;
Synapses/drug effects/metabolism/*physiology
;
Synaptic Transmission/drug effects/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
