Publication Date:
2013-11-16
Description:
Rapid and reductive cell divisions during embryogenesis require that intracellular structures adapt to a wide range of cell sizes. The mitotic spindle presents a central example of this flexibility, scaling with the dimensions of the cell to mediate accurate chromosome segregation. To determine whether spindle size regulation is achieved through a developmental program or is intrinsically specified by cell size or shape, we developed a system to encapsulate cytoplasm from Xenopus eggs and embryos inside cell-like compartments of defined sizes. Spindle size was observed to shrink with decreasing compartment size, similar to what occurs during early embryogenesis, and this scaling trend depended on compartment volume rather than shape. Thus, the amount of cytoplasmic material provides a mechanism for regulating the size of intracellular structures.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094345/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094345/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Good, Matthew C -- Vahey, Michael D -- Skandarajah, Arunan -- Fletcher, Daniel A -- Heald, Rebecca -- GM074751/GM/NIGMS NIH HHS/ -- GM098766/GM/NIGMS NIH HHS/ -- R01 GM074751/GM/NIGMS NIH HHS/ -- R01 GM098766/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2013 Nov 15;342(6160):856-60. doi: 10.1126/science.1243147.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cellular Biology, University of California-Berkeley, Berkeley, CA 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24233724" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Division
;
Cell Size
;
Cytoplasm/chemistry/*physiology/ultrastructure
;
*Embryonic Development
;
Ovum
;
Spindle Apparatus/chemistry/*physiology/ultrastructure
;
Xenopus laevis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
