Publication Date:
2013-07-28
Description:
A systematic, efficient means of producing diverse libraries of asymmetrically branched N-glycans is needed to investigate the specificities and biology of glycan-binding proteins. To that end, we describe a core pentasaccharide that at potential branching positions is modified by orthogonal protecting groups to allow selective attachment of specific saccharide moieties by chemical glycosylation. The appendages were selected so that the antenna of the resulting deprotected compounds could be selectively extended by glycosyltransferases to give libraries of asymmetrical multi-antennary glycans. The power of the methodology was demonstrated by the preparation of a series of complex oligosaccharides that were printed as microarrays and screened for binding to lectins and influenza-virus hemagglutinins, which showed that recognition is modulated by presentation of minimal epitopes in the context of complex N-glycans.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826785/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826785/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wang, Zhen -- Chinoy, Zoeisha S -- Ambre, Shailesh G -- Peng, Wenjie -- McBride, Ryan -- de Vries, Robert P -- Glushka, John -- Paulson, James C -- Boons, Geert-Jan -- AI058113/AI/NIAID NIH HHS/ -- P01 AI058113/AI/NIAID NIH HHS/ -- P41 RR005351/RR/NCRR NIH HHS/ -- P41GM103390/GM/NIGMS NIH HHS/ -- P41RR005351/RR/NCRR NIH HHS/ -- R01 GM090269/GM/NIGMS NIH HHS/ -- R01GM090269/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2013 Jul 26;341(6144):379-83. doi: 10.1126/science.1236231.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23888036" target="_blank"〉PubMed〈/a〉
Keywords:
Binding Sites
;
Carbohydrate Conformation
;
Carbohydrate Sequence
;
Epitopes
;
Glycosylation
;
Glycosyltransferases/*metabolism
;
Hemagglutinin Glycoproteins, Influenza Virus/chemistry/*metabolism
;
Lectins/chemistry/*metabolism
;
Mass Spectrometry
;
Microarray Analysis
;
Nuclear Magnetic Resonance, Biomolecular
;
Oligosaccharides/biosynthesis/*chemical synthesis/*chemistry/metabolism
;
Plant Lectins/chemistry/metabolism
;
Ribosome Inactivating Proteins/chemistry/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
