Publication Date:
2012-06-23
Description:
Transcription factors (TFs) are proteins that regulate the expression of genes by binding sequence-specific sites on the chromosome. It has been proposed that to find these sites fast and accurately, TFs combine one-dimensional (1D) sliding on DNA with 3D diffusion in the cytoplasm. This facilitated diffusion mechanism has been demonstrated in vitro, but it has not been shown experimentally to be exploited in living cells. We have developed a single-molecule assay that allows us to investigate the sliding process in living bacteria. Here we show that the lac repressor slides 45 +/- 10 base pairs on chromosomal DNA and that sliding can be obstructed by other DNA-bound proteins near the operator. Furthermore, the repressor frequently (〉90%) slides over its natural lacO(1) operator several times before binding. This suggests a trade-off between rapid search on nonspecific sequences and fast binding at the specific sequence.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hammar, Petter -- Leroy, Prune -- Mahmutovic, Anel -- Marklund, Erik G -- Berg, Otto G -- Elf, Johan -- New York, N.Y. -- Science. 2012 Jun 22;336(6088):1595-8. doi: 10.1126/science.1221648.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22723426" target="_blank"〉PubMed〈/a〉
Keywords:
Binding Sites
;
Chromosomes, Bacterial/metabolism
;
DNA, Bacterial/*metabolism
;
Escherichia coli/genetics/*metabolism
;
Escherichia coli Proteins/*metabolism
;
Facilitated Diffusion
;
Kinetics
;
*Lac Operon
;
Lac Repressors/*metabolism
;
*Operator Regions, Genetic
;
Protein Binding
;
Transcription Factors/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
