Publication Date:
2011-04-02
Description:
Waves of cyclin synthesis and degradation regulate the activity of Cdc2 protein kinase during the cell cycle. Cdc2 inactivation by Wee1B-mediated phosphorylation is necessary for arrest of the oocyte at G2-prophase, but it is unclear whether this regulation functions later during the metaphase-to-anaphase transition. We show that reactivation of a Wee1B pathway triggers the decrease in Cdc2 activity during egg activation. When Wee1B is down-regulated, oocytes fail to form a pronucleus in response to Ca(2+) signals. Calcium-calmodulin-dependent kinase II (CaMKII) activates Wee1B, and CaMKII-driven exit from metaphase II is inhibited by Wee1B down-regulation, demonstrating that exit from metaphase requires not only a proteolytic degradation of cyclin B but also the inhibitory phosphorylation of Cdc2 by Wee1B.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104668/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104668/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Oh, Jeong Su -- Susor, Andrej -- Conti, Marco -- GM080527-05/GM/NIGMS NIH HHS/ -- HD052909/HD/NICHD NIH HHS/ -- R01 GM080527/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2011 Apr 22;332(6028):462-5. doi: 10.1126/science.1199211. Epub 2011 Mar 31.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143-0556, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21454751" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
CDC2 Protein Kinase/antagonists & inhibitors/metabolism
;
Calcium/metabolism
;
Calcium Signaling
;
Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
;
Cell Cycle Proteins/genetics/*metabolism
;
Cyclin B/genetics/metabolism
;
Down-Regulation
;
Female
;
Gene Knockdown Techniques
;
Maturation-Promoting Factor/metabolism
;
*Meiosis
;
*Metaphase
;
Mice
;
Mice, Inbred C57BL
;
Oocytes/*physiology
;
Phosphorylation
;
Protein-Tyrosine Kinases/genetics/*metabolism
;
RNA, Messenger/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
