Publication Date:
2008-03-01
Description:
Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of intraluminal vesicles are unknown. Here, we find that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of the endosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide. Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral sphingomyelinases. These results establish a pathway in intraendosomal membrane transport and exosome formation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Trajkovic, Katarina -- Hsu, Chieh -- Chiantia, Salvatore -- Rajendran, Lawrence -- Wenzel, Dirk -- Wieland, Felix -- Schwille, Petra -- Brugger, Britta -- Simons, Mikael -- New York, N.Y. -- Science. 2008 Feb 29;319(5867):1244-7. doi: 10.1126/science.1153124.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centre for Biochemistry and Molecular Cell Biology, University of Gottingen, 37073 Gottingen, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18309083" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line
;
Cell Line, Tumor
;
Ceramides/analysis/*metabolism
;
Cytoplasmic Vesicles/chemistry/*metabolism/ultrastructure
;
Endosomes/*metabolism/ultrastructure
;
Humans
;
Intracellular Membranes/*metabolism/ultrastructure
;
Membrane Microdomains/*metabolism/ultrastructure
;
Mice
;
Myelin Proteolipid Protein/*metabolism
;
Oligodendroglia/metabolism/ultrastructure
;
Protein Transport
;
Receptor, Epidermal Growth Factor/metabolism
;
Recombinant Fusion Proteins/metabolism
;
Sphingomyelin Phosphodiesterase/antagonists & inhibitors/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
