Publication Date:
2007-02-10
Description:
Zhang et al. (Research Articles, 11 November 2005, p. 996) reported that obestatin, a peptide derived from the ghrelin precursor, activated the orphan G protein-coupled receptor GPR39. However, we found that I125-obestatin does not bind GPR39 and observed no effects of obestatin on GPR39-transfected cells in various functional assays (cyclic adenosine monophosphate production, calcium mobilization, and GPR39 internalization). Our results indicate that obestatin is not the cognate ligand for GPR39.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chartrel, N -- Alvear-Perez, R -- Leprince, J -- Iturrioz, X -- Reaux-Le Goazigo, A -- Audinot, V -- Chomarat, P -- Coge, F -- Nosjean, O -- Rodriguez, M -- Galizzi, J P -- Boutin, J A -- Vaudry, H -- Llorens-Cortes, C -- New York, N.Y. -- Science. 2007 Feb 9;315(5813):766; author reply 766.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut National de la Sante et de la Recherche Medicale (INSERM), U413, Laboratory of Cellular and Molecular Neuroendocrinology, and European Institute for Peptide Research (IFRMP 23), University of Rouen, 76821 Mont-Saint-Aignan, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17289961" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
CHO Cells
;
Calcium/metabolism
;
Cell Membrane/metabolism
;
Colforsin/pharmacology
;
Cricetinae
;
Cricetulus
;
Cyclic AMP/metabolism
;
Ghrelin
;
Humans
;
Ligands
;
Molecular Sequence Data
;
Peptide Hormones/genetics/*metabolism/pharmacology
;
Pituitary Gland/cytology/metabolism
;
Protein Binding
;
Receptors, G-Protein-Coupled/genetics/*metabolism
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
