Publication Date:
2004-01-13
Description:
The imprinted regulation of H19 and Insulin-like growth factor 2 expression involves binding of the vertebrate insulator protein, CCCTC binding factor (CTCF), to the maternally hypomethylated differentially methylated domain (DMD). How this hypomethylated state is maintained during oogenesis and the role of CTCF, if any, in this process are not understood. With the use of a transgenic RNA interference (RNAi)-based approach to generate oocytes with reduced amounts of CTCF protein, we found increased methylation of the H19 DMD and decreased developmental competence of CTCF-deficient oocytes. Our results suggest that CTCF protects the H19 DMD from de novo methylation during oocyte growth and is required for normal preimplantation development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fedoriw, Andrew M -- Stein, Paula -- Svoboda, Petr -- Schultz, Richard M -- Bartolomei, Marisa S -- HD-42026/HD/NICHD NIH HHS/ -- T32 HD-07516/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 2004 Jan 9;303(5655):238-40.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia, PA 19104, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14716017" target="_blank"〉PubMed〈/a〉
Keywords:
Actins/genetics
;
Animals
;
DNA Methylation
;
DNA-Binding Proteins/genetics/*metabolism
;
Embryonic and Fetal Development
;
Female
;
Fertilization in Vitro
;
Gene Targeting
;
*Genomic Imprinting
;
Litter Size
;
Mice
;
Mice, Transgenic
;
Nuclear Proteins/genetics
;
Oocytes/*metabolism
;
*RNA Interference
;
RNA, Long Noncoding
;
RNA, Messenger/genetics/metabolism
;
RNA, Untranslated/*genetics
;
Repressor Proteins/genetics/*metabolism
;
Zygote/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
