Publication Date:
2003-08-02
Description:
Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the approximately 29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Alonso, Jose M -- Stepanova, Anna N -- Leisse, Thomas J -- Kim, Christopher J -- Chen, Huaming -- Shinn, Paul -- Stevenson, Denise K -- Zimmerman, Justin -- Barajas, Pascual -- Cheuk, Rosa -- Gadrinab, Carmelita -- Heller, Collen -- Jeske, Albert -- Koesema, Eric -- Meyers, Cristina C -- Parker, Holly -- Prednis, Lance -- Ansari, Yasser -- Choy, Nathan -- Deen, Hashim -- Geralt, Michael -- Hazari, Nisha -- Hom, Emily -- Karnes, Meagan -- Mulholland, Celene -- Ndubaku, Ral -- Schmidt, Ian -- Guzman, Plinio -- Aguilar-Henonin, Laura -- Schmid, Markus -- Weigel, Detlef -- Carter, David E -- Marchand, Trudy -- Risseeuw, Eddy -- Brogden, Debra -- Zeko, Albana -- Crosby, William L -- Berry, Charles C -- Ecker, Joseph R -- New York, N.Y. -- Science. 2003 Aug 1;301(5633):653-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12893945" target="_blank"〉PubMed〈/a〉
Keywords:
3' Untranslated Regions
;
5' Untranslated Regions
;
Alleles
;
Arabidopsis/*genetics/metabolism
;
Arabidopsis Proteins/genetics/metabolism
;
Base Composition
;
Chromosomes, Plant/genetics
;
DNA, Bacterial/genetics
;
DNA, Plant/chemistry/genetics
;
Ethylenes/pharmacology
;
Exons
;
Expressed Sequence Tags
;
Gene Expression
;
Gene Expression Profiling
;
Gene Expression Regulation, Plant/drug effects
;
Genes, Plant
;
*Genome, Plant
;
Introns
;
*Mutagenesis, Insertional
;
Mutation
;
Oligonucleotide Array Sequence Analysis
;
Promoter Regions, Genetic
;
Recombination, Genetic
;
Rhizobium/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
