Publication Date:
2002-10-05
Description:
In tropical Africa, Anopheles funestus is one of the three most important malaria vectors. We physically mapped 157 A. funestus complementary DNAs (cDNAs) to the polytene chromosomes of this species. Sequences of the cDNAs were mapped in silico to the A. gambiae genome as part of a comparative genomic study of synteny, gene order, and sequence conservation between A. funestus and A. gambiae. These species are in the same subgenus and diverged about as recently as humans and chimpanzees. Despite nearly perfect preservation of synteny, we found substantial shuffling of gene order along corresponding chromosome arms. Since the divergence of these species, at least 70 chromosomal inversions have been fixed, the highest rate of rearrangement of any eukaryote studied to date. The high incidence of paracentric inversions and limited colinearity suggests that locating genes in one anopheline species based on gene order in another may be limited to closely related taxa.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sharakhov, Igor V -- Serazin, Andrew C -- Grushko, Olga G -- Dana, Ali -- Lobo, Neil -- Hillenmeyer, Maureen E -- Westerman, Richard -- Romero-Severson, Jeanne -- Costantini, Carlo -- Sagnon, N'Fale -- Collins, Frank H -- Besansky, Nora J -- AI48842/AI/NIAID NIH HHS/ -- U01 AI48846/AI/NIAID NIH HHS/ -- U01 AI50687/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2002 Oct 4;298(5591):182-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Tropical Disease Research and Training, University of Notre Dame, Notre Dame, IN 46556-0369, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12364797" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Anopheles/classification/*genetics
;
*Chromosome Inversion
;
Chromosomes/genetics
;
Conserved Sequence
;
DNA, Complementary
;
Evolution, Molecular
;
Expressed Sequence Tags
;
*Gene Order
;
Gene Rearrangement
;
*Genes, Insect
;
Genetic Linkage
;
In Situ Hybridization, Fluorescence
;
Molecular Sequence Data
;
Mutation
;
Physical Chromosome Mapping
;
Species Specificity
;
Synteny
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
