Publication Date:
2001-07-14
Description:
The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hutvagner, G -- McLachlan, J -- Pasquinelli, A E -- Balint, E -- Tuschl, T -- Zamore, P D -- GM62862-01/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Aug 3;293(5531):834-8. Epub 2001 Jul 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11452083" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blotting, Northern
;
Drosophila melanogaster
;
Endoribonucleases/genetics/*metabolism
;
*Gene Expression Regulation, Developmental
;
HeLa Cells
;
Humans
;
Nucleic Acid Conformation
;
Protein Structure, Tertiary
;
RNA Precursors/*metabolism
;
RNA Processing, Post-Transcriptional
;
RNA, Double-Stranded/*metabolism
;
RNA, Helminth/chemistry/genetics/*metabolism
;
RNA, Messenger/genetics/metabolism
;
Ribonuclease III
;
Transcription, Genetic
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
