Publication Date:
2009-11-13
Description:
Cohesin not only links sister chromatids but also inhibits the transcriptional machinery's interaction with and movement along chromatin. In contrast, replication forks must traverse such cohesin-associated obstructions to duplicate the entire genome in S phase. How this occurs is unknown. Through single-molecule analysis, we demonstrate that the replication factor C (RFC)-CTF18 clamp loader (RFC(CTF18)) controls the velocity, spacing and restart activity of replication forks in human cells and is required for robust acetylation of cohesin's SMC3 subunit and sister chromatid cohesion. Unexpectedly, we discovered that cohesin acetylation itself is a central determinant of fork processivity, as slow-moving replication forks were found in cells lacking the Eco1-related acetyltransferases ESCO1 or ESCO2 (refs 8-10) (including those derived from Roberts' syndrome patients, in whom ESCO2 is biallelically mutated) and in cells expressing a form of SMC3 that cannot be acetylated. This defect was a consequence of cohesin's hyperstable interaction with two regulatory cofactors, WAPL and PDS5A (refs 12, 13); removal of either cofactor allowed forks to progress rapidly without ESCO1, ESCO2, or RFC(CTF18). Our results show a novel mechanism for clamp-loader-dependent fork progression, mediated by the post-translational modification and structural remodelling of the cohesin ring. Loss of this regulatory mechanism leads to the spontaneous accrual of DNA damage and may contribute to the abnormalities of the Roberts' syndrome cohesinopathy.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777716/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777716/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Terret, Marie-Emilie -- Sherwood, Rebecca -- Rahman, Sadia -- Qin, Jun -- Jallepalli, Prasad V -- R01 CA107342/CA/NCI NIH HHS/ -- R01 CA107342-05/CA/NCI NIH HHS/ -- R01 GM094972/GM/NIGMS NIH HHS/ -- England -- Nature. 2009 Nov 12;462(7270):231-4. doi: 10.1038/nature08550.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19907496" target="_blank"〉PubMed〈/a〉
Keywords:
Acetylation
;
Acetyltransferases/deficiency/genetics
;
Carrier Proteins/genetics/metabolism
;
Cell Aging
;
Cell Cycle Proteins/chemistry/*metabolism
;
Cell Line
;
Chromatids/metabolism
;
Chromosomal Proteins, Non-Histone/chemistry/deficiency/genetics/*metabolism
;
DNA Damage
;
DNA Replication/drug effects/*physiology
;
Humans
;
Mutagens/toxicity
;
Nuclear Proteins/genetics/metabolism
;
Protein Subunits/metabolism
;
Proto-Oncogene Proteins/metabolism
;
Replication Protein C/metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
