Publication Date:
2016-04-24
Description:
TRP1 is a frequently used auxotrophic marker for genetic modifications and selections in trp – budding yeast strains, including the commonly used wild-type strain W303a. However, we found that introduction of the TRP1 gene into a trp – strain significantly affected vegetative growth at low and high temperatures. Therefore, caution should be needed when working in a trp – background strain and using the TRP1 marker to study stress response phenotypes, particularly when analyzing temperature sensitivities.
Keywords:
Physiology & Biochemistry
Print ISSN:
0378-1097
Electronic ISSN:
1574-6968
Topics:
Biology