Publication Date:
2014-04-15
Description:
Understanding the mechanism of Na + /K + -dependent spectral conversion of human telomeric G-quadruplex (G4) sequences has been limited not only because of the structural polymorphism but also the lack of sufficient structural information at different stages along the conversion process for one given oligonucleotide. In this work, we have determined the topology of the Na + form of Tel23 G4, which is the same hybrid form as the K + form of Tel23 G4 despite the distinct spectral patterns in their respective nuclear magnetic resonance (NMR) and circular dichroism spectra. The spectral difference, particularly the well-resolved imino proton NMR signals, allows us to monitor the structural conversion from Na + form to K + form during Na + /K + exchange. Time-resolved NMR experiments of hydrogen–deuterium exchange and hybridization clearly exclude involvement of the global unfolding for the fast Na + /K + spectral conversion. In addition, the K + titration monitored by NMR reveals that the Na + /K + exchange in Tel23 G4 is a two-step process. The addition of K + significantly stabilizes the unfolding kinetics of Tel23 G4. These results offer a possible explanation of rapid spectral conversion of Na + /K + exchange and insight into the mechanism of Na + /K + structural conversion in human telomeric G4s.
Print ISSN:
0305-1048
Electronic ISSN:
1362-4962
Topics:
Biology
