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Identification of sucrose synthase as an actin-binding protein (1998)

Huber, J. L. ; Davies, E. ; Huber, S. C. ; [et al.]

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Publication Date: 2011-08-24

Description: Several lines of evidence indicate that sucrose synthase (SuSy) binds both G- and F-actin: (i) presence of SuSy in the Triton X-100-insoluble fraction of microsomal membranes (i.e. crude cytoskeleton fraction); (ii) co-immunoprecipitation of actin with anti-SuSy monoclonal antibodies; (iii) association of SuSy with in situ phalloidin-stabilized F-actin filaments; and (iv) direct binding to F-actin, polymerized in vitro. Aldolase, well known to interact with F-actin, interfered with binding of SuSy, suggesting that a common or overlapping binding site may be involved. We postulate that some of the soluble SuSy in the cytosol may be associated with the actin cytoskeleton in vivo.

Keywords: Life Sciences (General)

Type: FEBS letters (ISSN 0014-5793); Volume 430; 3; 205-8

Format: text

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