ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A nonenzymatic spectrophotometric method, coupled to an automatic system of standard additions, based on the reaction with 3,5-dinitrosalicylic acid (DNSA) is described. In a system based on an exhaustive reaction, sample volumes are introduced together with calibration solutions, thus the calibration is performed in a nonsegmented flow system. Homogenization of the sample/standard/carrier takes place in the calibration loop. Later, it is injected in the flow injection analysis system, where the reaction with DNSA occurs, to obtain 3,5-diaminosalicylic acid which is measured spectrophotometrically. With the proposed method, it is possible to eliminate the Rochelle salt, sodium and potassium tartrate, phenol and sodium bisulfite of the principal reagent. Sample throughput was 11 samples/h and precision, expressed as relative standard deviation, was 2.3%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2001.tb16118.x
