ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
An Escherichia coli–Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp Eco RI digested fragment of pHLHK8 to Eco RI digested pBK-CMV. An E. coli–L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1016/j.femsle.2005.08.026