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Proteinase Inhibitors in Germinating Barley Embryos (1974)

KIRSI, MARKKU

Oxford, UK : Blackwell Publishing Ltd

Physiologia plantarum 32 (1974), S. 0

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ISSN: 1399-3054

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The activities of trypsin inhibitors and Aspergillus-proteinase inhibitors have been assayed in barley embryos germinating in different conditions.The Aspergillus-proteinase inhibitor activity increased about 20-fold during 6 days at 20°C both in separated embryos germinating on nutrient agar and in the embryos of undissected grains germinating on agar with or without mineral salts. Significant inhibitor activity was also present in root tips at later stages of vegetative growth. Whether any changes occurred in the molecular weight distribution of the inhibitors present in the seedlings was studied by gel filtration on Sephadex G-75; only minor changes were observed.The trypsin inhibitor activity per seedling remained roughly constant in all cultures. In gel filtration experiments all the activity appeared to be due to the inhibitor species present in resting embryos.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1399-3054.1974.tb03732.x

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