ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
A three-phase partitioning method to separate indolyl-3-acetic acid (IAA) and abscisic acid (ABA) from a crude plant extract was developed and evaluated. The aqueous phase at pH 2.7 of a methanolic plant extract constituted the 1st phase, from which IAA and ABA were transferred via diethyl ether, the 2nd phase, to a 3rd phase consisting of an alkaline buffer, enclosed in a dialysis tube. Partitioning of the free forms of the two hormones among the three phases in one container were carried out simultaneously and efficiently. The method also proved to be satisfactory when used as a combined step for both extraction and partitioning, at which the plant homogenate in buffer at pH 2.7 constituted the 1st phase. The content of IAA and ABA in kernels of Zea mays and in hypocotyls of Beta vulgaris were tested with the new method. The method presented is reliable and time-saving, and the demand for chemicals is less than for most of the conventional procedures used.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1983.tb02766.x
