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Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana (1995)

Joshi, Lokesh ; St. Leger, Raymond J. ; Bidochka, Michael J.

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 125 (1995), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract A Beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. A cDNA clone of the protease was isolated from mycelia of B. bassiana grown on cuticle/chitin cultures. The amino acid sequence of this gene was compared to that of Metarhizium anisopliae Pr1, the only pathogenicity determinant so far described from an entomopathogenic fungus, and proteinase K, isolated from Tritirachium album, a saprophytic fungus. The cDNA sequence revealed that B. bassiana Prl is synthesized as a large precursor (Mr 37 460) containing a signal peptide, a propeptide and the mature protein predicted to have an Mr of 26 832.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07360.x

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