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Cloning of the him genes encoding the integration host factor from Salmonella typhirmurium in E. coli (1987)

Raps, Helge ; Schumann, Wolfgang

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 48 (1987), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract By hybridization of himA and himD gene probes from E. coli to chromosomal DNA of Salmonella typhimurium cross-hybridization was obtained in both cases. A gene bank of Salmonella DNA was isolated using the mini-Mu cloning system. This gene bank was transformed into either a prototrophic E. coli himA or himD mutant. Transformants complementing either the himA or himD defect were isolated on minimal medium plates supplemented with 40 μg/ml leucin at 42°C. The Salmonella him genes on these plasmids were further verified by their ability to plate phage Mu and to yield turbid plaques with phage lambda and by the ability of the recombinant plasmids to hybridize to E. coli him gene probes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02625.x

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