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Acid-tolerant poly(3-hydroxybutyrate) hydrolases from moulds (1988)

McLellan, Douglas W. ; Halling, Peter J.

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 52 (1988), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract We have isolated some mould strains that can grow under acid conditions with poly(3-hydroxybutyrate) (PHB) as sole carbon source, and secrete PHB hydrolases active at pH values at least down to 3. An improved assay method for such enzymes using a pH stat has been developed, and used to determine the dependence of reaction rate on enzyme and polymer concentrations. The implications of these kinetic properties of the PHB hydrolase for its mode of action are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02598.x

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