ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Arabinose and galactose were detected in purified type G botulinum toxin (Mr about 500 000) of Clostridium argentinense. The i.p. LD50/mg N of type G progenitor toxin was one-tenth, but the oral LD50/mg N twice that of type A-L toxin. The lysozyme-, endo-β-galactosidase-, and N-glucanase-treated toxins each had a molecular mass of about 300 000. The oral toxicity of the endo-β-galactosidase ot N-glucanase-treated toxin was one-fifth that of untreated progenitor toxin. On DEAE-Sephadex chromatography, the N-glucanase-treated toxin dissociated into two fractions, nontoxic and toxic. SDS-PAGE of the toxic fraction showed a single band with a Mr of about 1500 000, and after dithiothreitol treatment, two bands with Mr of 100 000 and 50 000.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1991.tb04522.x