ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The present study was designed to test the hypothesis that oxidative stress mediateschromium-induced cytotoxicity in MG63 cells and antioxidant N-acetyl-cysteine (NAC) canprovide protection for osteoblasts against chromium-induced oxidative stress. We assessed theeffects of chromium ions on cell viability, the level of intracellular reactive oxygen species (ROS)and intracellular ultrastructure in the presence or absence of NAC. A time- and concentrationdependentincreased cytotoxicity, intracellular ROS generation was found and intracellularultrastructure was damaged when cells were exposed to Cr+6. NAC afforded dose-dependentreduction to the cytotoxicity and level of cellular oxidative stress induced by Cr+6. Intracellularultrastructural alterations were reduced by the NAC pretreatment, too. Cr+3 had no significantlynegative influence in MG63 (5-20μM). Our results suggest that oxidative stress might be involvedin Cr+6 induced cytotoxicity in osteoblasts. NAC can play a critical role against Cr+6- inducedcytotoxicity. Cr+3 (5 -20μM) had no significant cytotoxicity in MG63 cells and cellular oxidativestress was not found, too
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.609.pdf
