ISSN:
1013-9826
Quelle:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Thema:
Maschinenbau
Notizen:
Mechano-active scaffolds were fabricated from very elasticpoly(L-lactide-co-ε-carprolactone). The scaffolds with 80 % porosity and 300~500 μm pore sizewere prepared by a gel-pressing method. As a control group for elastic properties of polymerscaffolds, rigid poly L-lactide scaffolds were fabricated. The scaffolds were seeded withchondrocytes and cultured to evaluate the effect of elastic properties of polymer scaffolds for thedifferentiation and the ECM secretion of chondrocytes. Also, the chondrocytes-seeded constructswere implanted in nude mice subcutaneously to investigate their biocompatibility and cartilageformation. From the biochemical analyses, chondrogenic differentiation was sustained andenhanced significantly and chondral extracellular matrix was increased through mechanicalstimulation of dynamic environment in the dynamic body systems. Histological analysis showedthat implants of PLCL constructs formed mature and well-developed cartilaginous tissue, asevidenced by chondrocytes within lacunae. Consequently, the elastic PLCL scaffolds could be usedto engineer cartilage in mechanically dynamic environments
Materialart:
Digitale Medien
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.405.pdf
