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Preparation and X-ray crystallographic analysis of recombinant obelin crystals diffracting to beyond 1.1 Å (2001)

Vysotski, Eugene S. ; Liu, Zhi Jie ; Rose, John ; [et al.]

Copenhagen : International Union of Crystallography (IUCr)

Acta crystallographica 57 (2001), S. 1919-1921

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ISSN: 1399-0047

Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001

Topics: Chemistry and Pharmacology , Geosciences , Physics

Notes: Crystals of recombinant obelin, the Ca2+-regulated photoprotein from the marine hydroid Obelia longissima, have been grown from a solution containing PEG 8000 and potassium phosphate. Hexaminecobalt trichloride was used as an additive to increase the chance of crystallization. The crystals grow in a light yellow cubic form (0.5 × 0.5 × 0.45 mm) which diffracts to beyond 1.1 Å resolution. The crystals belong to the space group C2, with unit-cell parameters a = 83.43, b = 54.92, c = 52.99 Å, β = 112.00°. The asymmetric unit contains one molecule. Crystals exposed to calcium ion before and after X-ray irradiation emit light, confirming that the crystals consist of an active photoprotein.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1107/S0907444901016523

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