ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The enzyme 3-dehydroquinase catalyzes the interconversion of 3-dehydroquinate and 3-dehydroshikimate. The enzymes are classified into two groups, type I and type II, which have different biochemical and biophysical properties and act with different mechanisms. The type II dehydroquinase of Helicobacter pylori, a dodecameric enzyme, was overexpressed in Escherichia coli. The recombinant protein has been crystallized at 296 K using polyethylene glycol (PEG) 4000 as a precipitant. Native X-ray diffraction data have been collected to 2.5 Å resolution using synchrotron radiation. The crystals are cubic and belong to the space group P4232, with unit-cell parameters a = b = c = 98.91 Å. The asymmetric unit contains one subunit of recombinant type II dehydroquinase, with a corresponding VM of 2.18 Å3 Da−1 and a solvent content of 43.6%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900016267
