ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Aminopropyl-silica sorbent
;
Continuous cleanup/preconcentration
;
Hydroxyvitamin D3 metabolites
;
Flow injection analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A new procedure for continuous cleanup and concentration of hydroxyvitamin D3 metabolites prior to their separation by HPLC and UV-detection is reported. The process is based on the use of aminopropyl-silica as solid-phase sorbent as an alternative to the use of nonpolar sorbents. The improvement thus achieved has been tested by comparing the results with those obtained using octadecyl-C18 as non-polar sorbent. The comparison has been based on the calibration graphs (linear range, detection and quantitation limits), precision and multiple standard addition method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02466466