ISSN:
1612-1112
Keywords:
Affinity chromatography
;
Anhydrochymotrypsin
;
Peptides with aromatic amino acids at C-termini
;
Diol silica
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Anhydrochymotrypsin (AHC), a catalytically inert derivative of chymotrypsin in which the serine-residue active site has been converted chemically to a dehydroalanine residue, was immobilized on diol silica by activation with trifluoroethanesulfonyl chloride. A AHC-diol-silica column was used for high-performance affinity chromatographic separation of peptides with aromatic amino acids at their C-termini from other peptides. Faster separations were achieved.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02275889