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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and culture of tobacco mesophyll protoplasts using a saline medium (1974)
    Springer
    Protoplasma 81 (1974), S. 363-372 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new method is proposed for the isolation of tobacco mesophyll protoplasts in which salts were used as the plasmolyticum instead of the traditionally applied sugars. With this method protoplasts were regularly obtained and practically independent of the physiological state of the plants. In addition, a salt medium was developed in which the majority of protoplasts dedifferenciate and divide at a developmental state in which no wall could be detected by plasmolysis. After transferring these cells into another medium they were able to regenerate a wall and to divide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281049
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