ISSN:
1435-0653
Keywords:
ASAP, allele-specific associated primers bp, base pairs cM, centimorgan NIL, near isogenic line RAPD, random amplified polymorphic DNA SCAR, sequence characterized amplified region
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Uromyces appendiculatus (Pers.) Unger, the causative fungus of rust in common beans (Phaseolus vulgaris L.), consists of many pathotypes or pathogenic races. Cultivar Ouro Negro is resistant to most pathotypes detected in Brazil in the last few decades. We attempted to identify molecular markers linked to a rust resistance gene block present in Ouro Negro. DNA samples extracted from homozygous near isogenic BC3F2:3 lines derived from a cross between susceptible cultivar US Pinto 111 and Ouro Negro were grouped, following greenhouse inoculations, into two contrasting bulks, one containing only resistant and the other only susceptible plants. The bulks were amplified with 605 random primers and two of them amplified bands which were heteromorphic between the two bulks. These random amplified polymorphic DNA (RAPD) bands were transformed into sequence characterized amplified regions (SCARs) SCARBA08 and SCARF10. The rust resistance–susceptibility phenotypes and the molecular genotypes with the two SCAR markers of 303 F2:3 families from US Pinto 111 × Ouro Negro were determined. SCARBA08 and SCARF10 were determined to be 4.3 ± 1.2 and 6.0 ± 1.3 centimorgans (cM) from the rust resistance locus, respectively. These markers are being used in combination with other markers for resistance to rust, anthracnose, and angular leaf spot previously identified in our laboratory to aid the indirect selection of desirable plants in segregating populations in which Ouro Negro has been used as donor parent.
Type of Medium:
Electronic Resource
