ISSN:
1435-0653
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Helianthus annuus L.) require large numbers for chromatographic analyses of half seeds. The objective of this research was to study the potential of near-infrared reflectance spectroscopy (NIRS) to estimate the oleic and linoleic acid concentrations of intact single sunflower achenes. Spectra from intact achenes were collected with a specially designed adapter and a standard monochromator instrument. Calibration equations for oleic and linoleic acid were developed from a calibration set including 465 intact achenes with a large range of variation for both fatty acids and were validated with an external set of 100 intact achenes. The coefficient of determination between NIRS and gas-liquid chromatography (GLC) was 0.88 for both fatty acids in external validation, demonstrating the high reliability of NIRS to predict oleic and linoleic acid concentrations of intact, single sunflower achenes. The calibration equation for oleic acid was used to evaluate two F2 populations segregating for this fatty acid, which were further analyzed by GLC. The use of NIRS predictions permitted the discrimination of the majority of the high oleic acid achenes (〉 700 g kg-1 ), demonstrating the use of NIRS for selecting sunflower achenes with specific oleic and linoleic acid concentrations.
Type of Medium:
Electronic Resource
