ISSN:
1432-1432
Keywords:
Key words: DNA repeats — DNA restriction fragment analysis — Taxonprint — Molecular systematics — Lacertidae — Primates
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. A specially optimized restriction analysis of highly repetitive DNA elements, called DNA taxonprint, was applied for phylogenetic study of primates and lizards. It was shown that electrophoretic bands of DNA repeats revealed by the taxonprint technique have valuable properties for molecular systematics. Approximately half of taxonprint bands (TB) are invariable and do not disappear from the genomes during evolution or change spontaneously. Presumably these invariable bands are restriction fragments of dispersed DNA repeats. Another group represents variable taxonprint bands that differ even between closely related species. These variable bands are probably represented by tandem DNA repeats and could be used as species-specific markers. It was shown that taxonprint bands are independent characters since the appearance of a new taxonprint band does not change the previous band pattern. Phylogenetic reconstruction carried out on taxonprint data demonstrated that this approach could be of general utility for molecular systematics and species identification.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00006446
