ISSN:
1432-1432
Keywords:
Phosphorylation
;
Prebiotic
;
Dinucleoside monophosphorothioate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Evaporation of a solution of thymidine plus either theexo or theendo diastereomer of uridine cyclic 2′,3′-O, O-phosphorothioate (U 〉 p(S) in 1,2-diaminoethane hydrochloride buffer gave the 2′,5′ and 3′,5′ isomers of (P-thio) uridylylthymidine (Up(S)dT) in a ratio of 1:2 with a combined yield of about 20%. These isomers were re-converted to U 〉 p(S) and dT by a reaction that is known to proceed by an in-line mechanism. Both the 2′,5′ and 3′,5′ isomers gave as product the same diastereomer of U 〉 p(S) that had been used originally in their formation. These dry-state ‘prebiotic’ reactions (Verlander, Lohrmann, and Orgel 1973) are thus shown to be stereospecific, and both the 2′,5′ and 3′,5′ internucleotide bonds are formed by an in-line mechanism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01731369
