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Gene expression of Bacillus subtilis subtilisin E in Thermus thermophilus (1999)

Takagi, H ; Suzumura, A ; Hoshino, T ; [et al.]

Springer

Journal of industrial microbiology and biotechnology 23 (1999), S. 214-217

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ISSN: 1476-5535

Keywords: Keywords: gene expression; subtilisin E; Thermus thermophilus

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The Bacillus subtilis subtilisin E gene was cloned into an expression vector of the extreme thermophile, Thermus thermophilus. Active subtilisin E was produced in E. coli, indicating that the Thermus promoter functions in E. coli. When the plasmid was further introduced into T. thermophilus, the subtilisin E gene was expressed and the gene product accumulated as an inactive pro-form, because the autoprocessing of the wild-type enzyme to the active-form did not occur at 50°C or above.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/sj.jim.2900722

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