ISSN:
1432-0878
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary 1. The technique of cultivation of dissociated neurons from chick spinal ganglia in Maximow assembly is described. 2. Three different techniques for preparation of neurons from spinal ganglia were compared: “hand-free dissection”, separation with nylon sieves and trypsinisation. All these techniques were useful. But the best results (working with 15 days chick embryo) were obtained by “hand-free dissection”. 3. The highest number of newly formed nerve processes was observed with spinal ganglia neurons from 12 to 16-day sold chick embryos. Under optimal conditions an average of 40% of the dissociated neurons formed processes. 4. Cells isolated from young (6 to 7-days old) embryos and embryos older than 19 days are not able to form new processes in culture, in our experimental conditions. 5. The influence of the composition of the medium in which the cells are cultivated was tested. A stimulatory effect on the outgrowth of new processes by 10–20% brain extract from 10-days chick embryos was demonstrated. The highest effect was observed with neurons isolated from 14 to 17-days old embryos. The presence of nerve growth factor (NGF) increased the number of processes newly formed in cultures from 8 to 17-days old embryos. With both brain extract and NGF, the length and ramifications of the fibres were greater.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00347031
