ISSN:
1617-4623
Keywords:
OmpR
;
EnvZ
;
Phosphorylation
;
Transcriptional control
;
DNA binding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The fadL gene of Escherichia coli codes for an outer membrane protein that is involved in the uptake of long-chain fatty acids. Uptake is regulated by environmental osmolarity, and decreases when the cells are grown under conditions of high osmolarity. A temperature-sensitive mutant that requires fatty acid for growth at 42° C was unable to grow at the high temperature even in the presence of fatty acid if the medium contained 10% sucrose. Promoter activity of the fadL gene in vivo was repressed by high osmolarity in a FadR repressor null mutant. Furthermore, in vitro transcription of the fadL gene was strongly repressed by the addition of OmpR and EnvZ proteins. The results of gel retardation and DNase I protection experiments indicated that OmpR, after incubation with the protein kinase EnvZ, specifically binds to at least four sites around the fadL promoter, two upstream and two downstream from the transcriptional start site. These results suggest that transcription of the fadL gene is osmotically regulated by the OmpREnvZ two-component system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00280384
